*switching to n00b mode* 
- SPTI or ASPI: you can check this in the IsoBuster settings (interface settings IIRC)
- what kind of software to burn discs have you installed? Any other than Nero and Alcohol?
What I suspect: ASPI or ASPI-like driver conflicts. That's because Nero always seems to install some HW-drivers, Alcohol also does this - furthermore a driver for a virtual drive is installed, the same goes for Daemon Tools.
Maybe you also want to open the image file with IsoBuster directly. If it can read the data then the image should be fine. Next thing I would try is creating another image (from another) disc and try the same (opening in IsoBuster and then mounting it with Daemon Tools). This way you check if it's a problem with this specific image - I doubt this, but better check this first.
Another thing you might want to try is mounting the image under linux, using some liveCD of your choice.
Or check if the problem goes away when deinstalling Nero + Alcohol and reinstalling DTools.
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As I posted above...:
Alright, so I got it installed and it said:
During mounting of the disc only a session/track layout could be found. No file-systems nor files and folders could be found.
Assuming you are looking for certain (missing) files, we suggest you run the option:
"Find missing files and folders"
Selecting 'Make it so' didn't seem to do anything, I got no error messages, but could see now files on the 'tree'. I'm not sure what this means.
After this, I did uninstall Nero/Alcohol/Daemon tools and tried the original disc and it did nothing. I also tried running the image with just Daemon Tools installed, and that did not work either.
How ya doing, buddy?